MA5-12308

antibody from Invitrogen Antibodies

Targeting: SLC5A5 NIS

Antibody data

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Validation data

Product number: MA5-12308 - Provider product page
Provider: Invitrogen Antibodies
Product name: SLC5A5 Monoclonal Antibody (FP5A)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA5-12308 targets Sodium Iodide Symporter in IHC (P) and WB applications and shows reactivity with Human samples.
Antibody clone number: FP5A
Concentration: 0.2 mg/mL

SLC5A5 protein structure - MA5-12308 shown in red.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 bHDACi Induces NIS Expression Specifically in BC Cells, but Not in Normal Breast or TC Cells (A) Graphs showing fold difference in NIS transcript levels for ZR-75-1, MCF-7, and ARO cell lines post-bHDACi treatment with respect to their untreated counterparts. Error bar represents SEM; significance determined by t test. *p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 AR-42 and MS-275 Enhance the NIS Promoter Activity and Expression In Vivo (A) Bioluminescence (BLI) images of representative mouse with orthotopic ZR-75-1 NF tumors showing change in NIS promoter activity in untreated control and AR-42 drug-treated groups. (B) Graph showing BLI signal quantified from control and treated groups, normalized to the respective signals before treatment. Error bar indicates SEM (n = 2).*p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 bHDACi Differentially Regulates NIS Expression across Organs In Vivo (A) Photomicrographs of NIS IHC performed on mouse thyroid, lung, ovary, stomach, and liver tissues harvested from untreated control or MS-275-treated mouse. DAB staining (brown) and respective H&E-stained images are displayed. (B) Histogram profiles of DAB-stained tissue sections showing NIS intensity profile obtained by using IHC Profiler program. (C) Images of gel picture showing amplified NIS transcripts as a measure of response to VPA and MS-275 drug treatment in various cancer cell lines, i.e., BC (ZR-75-1), lung cancer (A-549), hepatocellular carcinoma (HEPG2), ovarian cancer (A2780), and glioblastoma (U87). beta-actin housekeeping gene is used as loading control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 3 FIGURE Tsh, Tsh-R, and Nis protein levels in rats exposed to NaNO 3 . (A) Tsh levels measured in serum. (B) Tsh-R protein measured by Western blotting of the thyroid tissue. Molecular weight for TsrR is 87 kDa. (C) Nis protein measured by Western blotting of the thyroid tissue. Predicted molecular weight for Nis functional protein in thyroid is 87 kDa. As control was used beta Actin of 42 kDa. Data are shown as means +- SEM in groups of seven rats. Significant difference by Tukey's multiple comparisons test at p < .05

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Gene expression as an indicator of virus replication in non-human cell lines. ( a ) Rodent and porcine cells were infected with Ad5 and Ad5/3 viruses which express luciferase in a replication-dependent manner (Ad5DeltaE3-Luc and Ad5/3DeltaE3-Luc). Luc activity was determined on days 1, 2, 3 and 4 post infection. Hamster and porcine cells demonstrated time-dependent increase in Luc expression after Ad5 infection, denoting active replication. Only porcine cells showed time-dependent increase in Luc activity after Ad5/3 infection. Trend analysis across the cell lines shows significant difference in rate of change relative to porcine PK15 under same conditions (* p < 0.05; ** p < 0.005). ( b ) Immunofluorescent images of porcine PK15 cells infected with Ad5 and Ad5/3 vectors expressing replication-dependent sodium-iodide-symporter (NIS) and stained for Ad-hexon (green) and NIS (red) proteins, co-localization indicated in yellow (see arrows), mag. 20x. ( c ) Quantitative assessment by Flow Cytometry of PK15 porcine cells expressing NIS. From day 2 to day 5 the percentage of NIS-positive cells increased after infection with both Ad5DeltaE3-NIS and Ad5/3DeltaE3-NIS. The percentage of NIS-positive cells after infection with Ad5/3 was 4-fold greater than with Ad5 ( p < 0.05).