- GTX101206 - GeneTex FLNB antibody

Submitted references Mechanisms and Effects on HBV Replication of the Interaction between HBV Core Protein and Cellular Filamin B.

Li Y, Sun Y, Sun F, Hua R, Li C, Chen L, Guo D, Mu J
Virologica Sinica 2018 Apr;33(2):162-172

Filamin B Loss-of-Function Mutation in Dimerization Domain Causes Autosomal-Recessive Spondylocarpotarsal Synostosis Syndrome with Rib Anomalies.

Yang CF, Wang CH, Siong H'ng W, Chang CP, Lin WD, Chen YT, Wu JY, Tsai FJ
Human mutation 2017 May;38(5):540-547

Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network.

Artym VV, Swatkoski S, Matsumoto K, Campbell CB, Petrie RJ, Dimitriadis EK, Li X, Mueller SC, Bugge TH, Gucek M, Yamada KM
The Journal of cell biology 2015 Feb 2;208(3):331-50

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term (GTX101206) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 40548

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 5% SDS PAGE GTX101206 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A:NIH-3T3 5% SDS PAGE GTX101206 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: U87-MG 5% SDS PAGE GTX101206 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell extract 5% SDS-PAGEFilamin B antibody [N1], N-term (GTX101206) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody (GTX101206) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term (GTX101206) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B(GTX101206) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Filamin B antibody [N1], N-term (GTX101206) diluted at 1:500.

GTX101206

Antibody data