Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [10]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-90286 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90286, RRID:AB_11040692
- Product name
- Rabbit Polyclonal Filamin B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Filamin B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Thompson PJ, Shah A, Ntranos V, Van Gool F, Atkinson M, Bhushan A
Cell metabolism 2019 May 7;29(5):1045-1060.e10
Cell metabolism 2019 May 7;29(5):1045-1060.e10
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in human cell lines A-549 and HEK293 using anti-FLNB antibody. Corresponding FLNB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Analysis in human prostate and skeletal muscle tissues using. Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon, kidney, pancreas and testis using Anti-FLNB antibody NBP1-90286 (A) shows similar protein distribution across tissues to independent antibody NBP1-90285 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human pancreas.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human skin shows weak to moderate cytoplasmic positivity in keratinocytes.