H00001958-M03
antibody from Novus Biologicals
Targeting: EGR1
AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00001958-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001958-M03, RRID:AB_538484
- Product name
- Mouse Monoclonal EGR1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EGR1 - early growth response 1
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A novel EGR-1 dependent mechanism for YB-1 modulation of paclitaxel response in a triple negative breast cancer cell line.
Induction of hypertrophy in vitro by mechanical loading in adult rabbit myocardium.
Lasham A, Mehta SY, Fitzgerald SJ, Woolley AG, Hearn JI, Hurley DG, Ruza I, Algie M, Shelling AN, Braithwaite AW, Print CG
International journal of cancer 2016 Sep 1;139(5):1157-70
International journal of cancer 2016 Sep 1;139(5):1157-70
Induction of hypertrophy in vitro by mechanical loading in adult rabbit myocardium.
Bupha-Intr T, Holmes JW, Janssen PM
American journal of physiology. Heart and circulatory physiology 2007 Dec;293(6):H3759-67
American journal of physiology. Heart and circulatory physiology 2007 Dec;293(6):H3759-67
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of EGR1 expression in transfected 293T cell line by EGR1 monoclonal antibody (M03), clone 6E8. Lane 1: EGR1 transfected lysatE (59.73 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: EGR1 Antibody (6E8) [H00001958-M03] - Detection limit for recombinant GST tagged EGR1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml