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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-68143 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-68143, RRID:AB_1110229
- Product name
- Goat Polyclonal RPL8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
KAGRAYHKYKAKRNC- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.
Swoboda RK, Somasundaram R, Caputo L, Ochoa EM, Gimotty PA, Marincola FM, Van Belle P, Barth S, Elder D, Guerry D, Czerniecki B, Schuchter L, Vonderheide RH, Herlyn D
Cancer research 2007 Apr 15;67(8):3555-9
Cancer research 2007 Apr 15;67(8):3555-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPL8 Antibody [NB100-68143] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPL8 Antibody [NB100-68143] - Western blot analysis of RPL8 antibody using NB100-68143 at 0.01ug/ml in HepG2 cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143] - Staining of paraffin embedded Human Cerebral Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL8 Antibody [NB100-68143] - Staining of paraffin embedded Human Spleen. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
