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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB7307 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7307, RRID:AB_1580261
- Product name
- RPL8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of RPL8.
- Antigen sequence
KAGRAYHKYKAKRNC- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.
Swoboda RK, Somasundaram R, Caputo L, Ochoa EM, Gimotty PA, Marincola FM, Van Belle P, Barth S, Elder D, Guerry D, Czerniecki B, Schuchter L, Vonderheide RH, Herlyn D
Cancer research 2007 Apr 15;67(8):3555-9
Cancer research 2007 Apr 15;67(8):3555-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RPL8 polyclonal antibody (Cat # PAB7307) (0.01 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RPL8 polyclonal antibody (Cat # PAB7307) (2.5 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
