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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27467 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (98%), Japanese Medaka (97%), Drosophila (80%), Xenopus laevis (98%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.27 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL8 Polyclonal Antibody (Product # PA5-27467). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 12% SDS PAGE. RPL8 Polyclonal Antibody (Product # PA5-27467) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL8 Polyclonal Antibody (Product # PA5-27467). Sample (30 µg of whole cell lysate). Lane A: HepG2. Lane B: Molt-4. 12% SDS PAGE. RPL8 Polyclonal Antibody (Product # PA5-27467) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPL8 in methanol-fixed A431 cells using a RPL8 polyclonal antibody (Product # PA5-27467) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using RPL8 (Product # PA5-27467) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
