Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00009950-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009950-M01, RRID:AB_829059
- Product name
- Mouse Monoclonal GOLGA5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GOLGA5 (6B3)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01] - Analysis of GOLGA5 over-expressed 293 cell line, cotransfected with GOLGA5 Validated Chimera RNAi ( Cat # H00009950-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GOLGA5 monoclonal antibody (M01), clone 6B3 (Cat # H00009950-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01] - Analysis of GOLGA5 expression in transfected 293T cell line by GOLGA5 monoclonal antibody (M01), clone 6B3.Lane 1: GOLGA5 transfected lysate(83 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GOLGA5 Antibody (6B3) [H00009950-M01] - GOLGA5 monoclonal antibody (M01), clone 6B3 Analysis of GOLGA5 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: GOLGA5 Antibody (6B3) [H00009950-M01] - Detection limit for recombinant GST tagged GOLGA5 is approximately 0.1ng/ml as a capture antibody.