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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB9496-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human 5-HT6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human 5-HT6 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P50406- Isotype
- IgG
- Antibody clone number
- 2157A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- 5-HT6 in Rat Brain. 5-HT6 was detected in perfusion fixed frozen sections of rat brain (medulla) using Rabbit Anti-Human 5-HT6 Monoclonal Antibody (Catalog # MAB9496) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of neuronal cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
