Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 10886-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syndecan-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAIAYLGSS CPSQPPSSLA LSLSPTPSDF EQESGIETAM RFSPDVALAV STTPFEELPS ERPTLEPATS PLVVTEVPEE PSQRATTVST TMATTAATST GDPTVATVPA TVATATPSTP AAPPFTATTA VIRTTGVRRL LPLPLTTVAT ARATTPEAPS PPTTAAVLDT EAPTPRLVST ATSRPRALPR PATTQEPDIP ERSTLPLGTT APGPTEVAQT PTPETFLTTI RDEPEVPVSG GPSGDFELPE EETTQPDTAN EVVAVGGAAA KASSPPGTLP KGARPGPGLL DNAIDSGSSA AQLPQKSILE RKEVLVAVIV GGVVGALFAA FLVTLLIYRM KKKDEGSYTL EEPKQASVTY QKPDKQEEFY A (1-370 aa encoded by BC013974)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-12 cells using 10886-1-AP (Syndecan-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-12 cells using 10886-1-AP (Syndecan-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using SDC3 antibody 10886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10886-1-AP ( Syndecan-3 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10886-1-AP ( Syndecan-3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:1000) with mouse lung tissue lysate 4000ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Syndecan-3 (IP:10886-1-AP, 4 ug; Detection:10886-1-AP 1:600) with A549 cells lysate 2800 ug.