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antibody from Invitrogen Antibodies
Targeting: CX3CL1
ABCD-3, C3Xkine, CXC3, CXC3C, NTN, SCYD1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [3]
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- Product number
- 14-7986-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Description: The rabbit polyclonal antibody reacts with recombinant and natural mouse, rat, and human fractalkine (CX3CL1); the antibody was generated using E. coli-expressed chemotactic domain of human CX3CL1 as an immunogen. CX3CL1, also known as neurotactin or fractalkine, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain, the first two cysteine residues are separated by 3 amino acids (CX3C). CX3CL1 message is found in high abundance in the brain, kidney, lung and heart tissues. CX3CL1 is chemotactic for monocytes and other leukocytes including NK cells and may play a role in brain inflammation. Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CX3CL1 of other species has not been determined. Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows: Western Blot 1:1000-1:5000 Immunoprecipitation 1:300-1:800 Immunohistochemistry 1:100-1:500. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Fuzheng Huayu Recipe Prevented and Treated CCl4-Induced Mice Liver Fibrosis through Regulating Polarization and Chemotaxis of Intrahepatic Macrophages via CCL2 and CX3CL1.
Fractalkine shedding is mediated by p38 and the ADAM10 protease under pro-inflammatory conditions in human astrocytes.
MicroRNA-181b regulates NF-κB-mediated vascular inflammation.
Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells.
Dietary Aloe Improves Insulin Sensitivity via the Suppression of Obesity-induced Inflammation in Obese Mice.
Differential gene expression profiling of large and small retinal ganglion cells.
T cell costimulation by fractalkine-expressing synoviocytes in rheumatoid arthritis.
Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion.
Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation.
A new class of membrane-bound chemokine with a CX3C motif.
Zhang M, Liu HL, Huang K, Peng Y, Tao YY, Zhao CQ, Hu XD, Liu CH
Evidence-based complementary and alternative medicine : eCAM 2020;2020:8591892
Evidence-based complementary and alternative medicine : eCAM 2020;2020:8591892
Fractalkine shedding is mediated by p38 and the ADAM10 protease under pro-inflammatory conditions in human astrocytes.
O'Sullivan SA, Gasparini F, Mir AK, Dev KK
Journal of neuroinflammation 2016 Aug 22;13(1):189
Journal of neuroinflammation 2016 Aug 22;13(1):189
MicroRNA-181b regulates NF-κB-mediated vascular inflammation.
Sun X, Icli B, Wara AK, Belkin N, He S, Kobzik L, Hunninghake GM, Vera MP, MICU Registry, Blackwell TS, Baron RM, Feinberg MW
The Journal of clinical investigation 2012 Jun;122(6):1973-90
The Journal of clinical investigation 2012 Jun;122(6):1973-90
Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells.
Huang S, Zhao P, Yang L, Chen Y, Yan J, Duan E, Qiao J
Reproductive biology and endocrinology : RB&E 2011 Jun 30;9:95
Reproductive biology and endocrinology : RB&E 2011 Jun 30;9:95
Dietary Aloe Improves Insulin Sensitivity via the Suppression of Obesity-induced Inflammation in Obese Mice.
Shin E, Shim KS, Kong H, Lee S, Shin S, Kwon J, Jo TH, Park YI, Lee CK, Kim K
Immune network 2011 Feb;11(1):59-67
Immune network 2011 Feb;11(1):59-67
Differential gene expression profiling of large and small retinal ganglion cells.
Ivanov D, Dvoriantchikova G, Barakat DJ, Nathanson L, Shestopalov VI
Journal of neuroscience methods 2008 Sep 15;174(1):10-7
Journal of neuroscience methods 2008 Sep 15;174(1):10-7
T cell costimulation by fractalkine-expressing synoviocytes in rheumatoid arthritis.
Sawai H, Park YW, Roberson J, Imai T, Goronzy JJ, Weyand CM
Arthritis and rheumatism 2005 May;52(5):1392-401
Arthritis and rheumatism 2005 May;52(5):1392-401
Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion.
Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki M, Takagi S, Nomiyama H, Schall TJ, Yoshie O
Cell 1997 Nov 14;91(4):521-30
Cell 1997 Nov 14;91(4):521-30
Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation.
Pan Y, Lloyd C, Zhou H, Dolich S, Deeds J, Gonzalo JA, Vath J, Gosselin M, Ma J, Dussault B, Woolf E, Alperin G, Culpepper J, Gutierrez-Ramos JC, Gearing D
Nature 1997 Jun 5;387(6633):611-7
Nature 1997 Jun 5;387(6633):611-7
A new class of membrane-bound chemokine with a CX3C motif.
Bazan JF, Bacon KB, Hardiman G, Wang W, Soo K, Rossi D, Greaves DR, Zlotnik A, Schall TJ
Nature 1997 Feb 13;385(6617):640-4
Nature 1997 Feb 13;385(6617):640-4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™ (Product # 14-7986-81) and a 85 kDa band corresponding to soluble cleaved form of CX3CL1 (Fractalkine) was observed across tested cell line. Conditioned media (20 µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF-alpha, 10 ng/ml for 12 hrs (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Soluble fractalkine (sCX3CL1) release from human astrocytes. a Human astrocytes (grown in speciality media) were treated with IL-1beta (1 pg/ml, 10 pg/ml and 100 pg/ml) for 6 or 18 h, and the levels of sCX3CL1 in the media were quantified by ELISA. The levels of CX3CL1 were significantly higher after 18-h treatments compared to 6 h. All values expressed as averages +- SEM; n = 4, each condition done in duplicate. One-way ANOVA and Tukey's post hoc test; *** p < 0.001 compared to control. b Immunostaining of up-regulated CX3CL1 on human astrocytes (grown in speciality media) when treated with IL-1beta (100 pg/ml), TNF-alpha (10 ng/ml) and IFN-gamma (10 ng/ml) for 18 h. c Quantification of total cellular content of CX3CL1 following cytokine stimulation. Human astrocytes (grown in standard media) were treated with a IL-1beta (100 pg/ml), TNF-alpha (10 ng/ml) and IFN-gamma (10 ng/ml) for 18 h. Following stimulation, cells were lysed with PTxE lysis buffer and the levels of sCX3CL1 were quantified by ELISA. Data presented as mean +- SEM, n = 4, unpaired t test, * p < 0.05, *** p < 0.001 vs. corresponding control. In all cases, cells were serum starved 3 h before treatments
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 FZHY could regulate liver inflammatory cytokines and chemokines in liver tissue during liver fibrosis. (a) Immunofluorescence, (b, d) Western blot, and (c, e) qRT-PCR. Results are expressed as mean +- SD ( n = 3). # p < 0.05 and ## p < 0.01 vs. normal group, * p < 0.05 and ** p < 0.01 vs. CCl4 group.
