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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- AF1161 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Livin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Livin in Western blots. Can detect recombinant and endogenous human Livin alpha (KIAP) and Livin beta (ML-IAP).
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q96CA5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
Engesæter B, Engebraaten O, Flørenes VA, Mælandsmo GM
PloS one 2012;7(9):e45492
PloS one 2012;7(9):e45492
Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
Toosi S, Habib N, Torres G, Reynolds SR, Bystryn JC
The Journal of investigative dermatology 2011 Nov;131(11):2327-9
The Journal of investigative dermatology 2011 Nov;131(11):2327-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Livin by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, Recombinant Human Livin alpha/KIAP (Catalog # 1161-LV) and Recombinant Human Livin beta/ML-IAP (Catalog # 787-LV) (0.5 ng/lane) were included. A specific band for Livin was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Livin by Simple WesternTM. Simple Western lane view shows lysates of SK-Mel-28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Livin at approximately 42 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
