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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunocytochemistry [3]
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- Product number
- ALX-804-508-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-508-C100, RRID:AB_2050862
- Product name
- Bmf (mouse/rat) monoclonal antibody (17A9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 17A9
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references BH3-only protein Bmf mediates apoptosis upon inhibition of CAP-dependent protein synthesis.
BID, BIM, and PUMA are essential for activation of the BAX- and BAK-dependent cell death program.
The role of BH3-only protein Bim extends beyond inhibiting Bcl-2-like prosurvival proteins.
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development.
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
Grespi F, Soratroi C, Krumschnabel G, Sohm B, Ploner C, Geley S, Hengst L, Häcker G, Villunger A
Cell death and differentiation 2010 Nov;17(11):1672-83
Cell death and differentiation 2010 Nov;17(11):1672-83
BID, BIM, and PUMA are essential for activation of the BAX- and BAK-dependent cell death program.
Ren D, Tu HC, Kim H, Wang GX, Bean GR, Takeuchi O, Jeffers JR, Zambetti GP, Hsieh JJ, Cheng EH
Science (New York, N.Y.) 2010 Dec 3;330(6009):1390-3
Science (New York, N.Y.) 2010 Dec 3;330(6009):1390-3
The role of BH3-only protein Bim extends beyond inhibiting Bcl-2-like prosurvival proteins.
Mérino D, Giam M, Hughes PD, Siggs OM, Heger K, O'Reilly LA, Adams JM, Strasser A, Lee EF, Fairlie WD, Bouillet P
The Journal of cell biology 2009 Aug 10;186(3):355-62
The Journal of cell biology 2009 Aug 10;186(3):355-62
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development.
Labi V, Erlacher M, Kiessling S, Manzl C, Frenzel A, O'Reilly L, Strasser A, Villunger A
The Journal of experimental medicine 2008 Mar 17;205(3):641-55
The Journal of experimental medicine 2008 Mar 17;205(3):641-55
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
Ying S, Seiffert BM, Häcker G, Fischer SF
Infection and immunity 2005 Mar;73(3):1399-403
Infection and immunity 2005 Mar;73(3):1399-403
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- A) Detection of overexpressed Bmf in 293T cells transiently transfected with mouse Bmfby Western blot using MAb to Bmf (17A9) (Prod. No. ALX-804-508). . B) Detection of endogenous Bmf by Western blot using MAb to Bmf (17A9) (Prod. No. ALX-804-508) in mouse spleen, lymph node and thymus as two bands of ~25 and ~30kDa, but not in these tissues derived from two knock-out Bmf strains del 372 and del 376.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- C) Detection of mouse and rat Bmf by Western blot using MAb to Bmf (17A9) (Prod. No. ALX-804-508). . D) Immunoprecipitation of endogenous Bmf from mouse spleen cells using MAb to Bmf (17A9) (Prod. No. ALX-804-508).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Flow cytometry data of 293T cells transiently transfected with EE-tagged Bmf, fixed with 1% PFA, permeabilised with saponin and stained intracellularly with Bmf (mouse/rat), mAb (17A9) (Prod. No. ALX-804-508) or positive control anti-EE.
