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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB10099-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat PGD2 Synthase/PTGDS Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and rat PGD2 Synthase/PTGDS in direct ELISA and Western blots.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P41222- Isotype
- IgG
- Antibody clone number
- 997303
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat PGD2 Synthase/PTGDS by Western Blot. Western blot shows lysates of human heart tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PGD2 Synthase/PTGDS at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PGD2 Synthase/PTGDS in Human Brain. PGD2 Synthase/PTGDS was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
