- MA5-34642 - Invitrogen Antibodies MATR3 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Matrin 3 in Lane 1: Daudi, Lane 2: Mouse brain tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot of Matrin-3 was performed by loading 20 µg of WT (lane 1) and MATR3 CRISPR KO (lane 2) HAP1 cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. MATR3 was detected above 100 kDa (designated by the black arrow) using a MATR3 recombinant monoclonal antibody (Product # MA5-34642) at a dilution of 1:1,000 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 65-6120) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Matrin 3 in Lane 1: Daudi, Lane 2: Mouse brain tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence of MATR3 was performed using HAP1 wild-type and MATR3 KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the MATR3 recombinant monoclonal antibody (Product # MA5-34642) at a 1:1,000 dilution overnight at 4°C. Secondary antibody incubation was performed using 1 µg/mL of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 antibody (Product # A21429) for 1 hr. Imaging was performed with a 40X oil objective and analysis was performed using Image J. Cell image represents a single focal plane; WT and KO cells are outlined with a yellow (WT) or magenta (KO) dashed line. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Matrin 3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Matrin 3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Matrin 3 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of Matrin 3 in paraffin-embedded rat brain tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of Matrin 3 in paraffin-embedded mouse testis tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of Matrin 3 in paraffin-embedded human tonsil tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of Matrin 3 in paraffin-embedded human lung cancer tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Matrin 3 in paraffin-embedded human liver tissue. Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry of Matrin 3 in LOVO cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Matrin 3 monoclonal antibody (Product # MA5-34642) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

MA5-34642