H00025803-M01

antibody from Abnova Corporation

Targeting: SPDEF bA375E1.3, PDEF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00025803-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00025803-M01, RRID:AB_519063

Product name

SPDEF monoclonal antibody (M01), clone 4A5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SPDEF.

Antigen sequence

QCPVIDSQAPAGSLDLVPGGLTLEEHSLEQVQSMV
VGEVLKDIETACKLLNITADPMDWSPSNVQKWLLW
TEHQYRLPPMGKAFQELAGKELCAMSEEQFRQRSP
LGGD


Isotype

IgG

Antibody clone number

4A5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.Lane 1: SPDEF transfected lysate(37.518 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SPDEF is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol