ABIN1109117

antibody from antibodies-online

Targeting: SPDEF bA375E1.3, PDEF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1109117

Provider

antibodies-online

Product name

anti-Proximal Sequence Element 1 (N-Term) antibody

Antibody type

Polyclonal

Antigen

The immunogen for anti-SPDEF antibody: synthetic peptide directed towards the N terminal of human SPDEF.

Description

Purified on Protein A affinity column

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Rabbit

Antigen sequence

AAAGAVGLERRDWSPSPPATPEQGLSAFYLSYFDM
LYPEDSSWAAKAPGA


Epitope

N-Term

Vial size

0.1 mg

Storage

Store the lyophised antibody at -20°C for up to one year. Store reconstitued antibody undiluted for one month or in aliquots at -20°C long term.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

RUNX1 (AML-1) and RUNX2 (AML-3) cooperate with prostate-derived Ets factor to activate transcription from the PSA upstream regulatory region.

Fowler M, Borazanci E, McGhee L, Pylant SW, Williams BJ, Glass J, Davis JN, Meyers S
Journal of cellular biochemistry 2006 Jan 1;97(1):1-17

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Human MCF7; WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate. SPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SPDEF antibody - N-terminal region (AP42057PU-N) in Human MCF7 cells using Western Blot