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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA500990 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500990, RRID:AB_11125965
- Product name
- Anti-MCL1 mouse monoclonal antibody, clone OTI10D10 (formerly 10D10)
- Antibody type
- Monoclonal
- Description
- Anti-MCL1 mouse monoclonal antibody, clone OTI10D10 (formerly 10D10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MCL1
- Isotype
- IgG
- Antibody clone number
- OTI10D10
- Vial size
- 100 µl
- Concentration
- 0.95 mg/ml
Submitted references The combination of Nutlin-3 and Tanshinone IIA promotes synergistic cytotoxicity in acute leukemic cells expressing wild-type p53 by co-regulating MDM2-P53 and the AKT/mTOR pathway.
Guo Y, Li Y, Wang FF, Xiang B, Huang XO, Ma HB, Gong YP
The international journal of biochemistry & cell biology 2019 Jan;106:8-20
The international journal of biochemistry & cell biology 2019 Jan;106:8-20
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-MCL1 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-MCL1 mouse monoclonal antibody (TA500990) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1(RC200521).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500990, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500990, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY MCL1(RC200521)(Red) or empty vector control plasmid(Blue) were immunostained with anti-MCL1 mouse monoclonal(TA500990), and then analyzed by flow cytometry.
- Validation comment
- FC
