AF8281

antibody from R&D Systems

Targeting: MCL1 BCL2L3, Mcl-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF8281

Provider

R&D Systems

Product name

Human Mcl-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Mcl-1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q07820


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Mcl-1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Mcl-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8281) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mcl-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human Mcl-1 Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Mcl-1 knockout A431 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Mcl-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8281) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mcl-1 at approximately 40 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.