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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AF513 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal MMP-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and primate MMP-3 in ELISAs and Western blots. In sandwich ELISAs, less than 2.5% cross-reactivity with recombinant human (rh) MMP-10 is observed and less than 0.1% cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, -13, and recombinant mouse MMP-9 is observed.
- Reactivity
- Human, Simian
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
IL-3 Decreases Cartilage Degeneration by Downregulating Matrix Metalloproteinases and Reduces Joint Destruction in Osteoarthritic Mice.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
Molecular profiling of cervical cancer progression.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Beltrami-Moreira M, Vromman A, Sukhova GK, Folco EJ, Libby P
PloS one 2016;11(3):e0152474
PloS one 2016;11(3):e0152474
IL-3 Decreases Cartilage Degeneration by Downregulating Matrix Metalloproteinases and Reduces Joint Destruction in Osteoarthritic Mice.
Kour S, Garimella MG, Shiroor DA, Mhaske ST, Joshi SR, Singh K, Pal S, Mittal M, Krishnan HB, Chattopadhyay N, Ulemale AH, Wani MR
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 15;196(12):5024-35
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 15;196(12):5024-35
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
Life sciences 2008 Jun 6;82(23-24):1203-9
Life sciences 2008 Jun 6;82(23-24):1203-9
Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
FEBS letters 2007 May 1;581(9):1928-32
FEBS letters 2007 May 1;581(9):1928-32
Molecular profiling of cervical cancer progression.
Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
British journal of cancer 2007 Jan 29;96(2):321-8
British journal of cancer 2007 Jan 29;96(2):321-8
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human MMP-3 by Western Blot. Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (Catalog # WBC015) and lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MMP-3 in Human Bladder Cancer Tissue. MMP-3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human/ Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MMP-3 in Human Lung Cancer. MMP-3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
