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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21600 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOCS5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Molt-4, NT2D1, PC-3, mouse testis.
- Concentration
- 1 mg/mL
Submitted references Maintenance of cancer stemness by miR-196b-5p contributes to chemoresistance of colorectal cancer cells via activating STAT3 signaling pathway.
Ren D, Lin B, Zhang X, Peng Y, Ye Z, Ma Y, Liang Y, Cao L, Li X, Li R, Sun L, Liu Q, Wu J, Zhou K, Zeng J
Oncotarget 2017 Jul 25;8(30):49807-49823
Oncotarget 2017 Jul 25;8(30):49807-49823
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOCS5 Polyclonal Antibody (Product # PA5-21600). Sample (30 µg of whole cell lysate). Lane A: Molt-4 . 7.5% SDS PAGE. SOCS5 Polyclonal Antibody . SOCS5 Polyclonal Antibody (Product # PA5-21600) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOCS5 Polyclonal Antibody detects SOCS5 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract. B. 30 µg PC-3 whole cell lysate/extract.7.5 % SDS-PAGE. SOCS5 Polyclonal Antibody (Product # PA5-21600) dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOCS5 Polyclonal Antibody detects SOCS5 protein by Western blot analysis. A. 50 µg mouse testis lysate/extract.7.5 % SDS-PAGE. SOCS5 Polyclonal Antibody (Product # PA5-21600) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using SOCS5 (Product # PA5-21600) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 miR-196b-5p activates STAT3 signaling via targeting multiple negative regulators of STAT3 signaling ( A ) Real-time PCR analysis of miR-196b-5p expression in the indicated cells. Transcript levels were normalized by U6 expression. Error bars represent the mean +- SD of three independent experiments. * P < 0.05. ( B ) Western blotting of SOCS1, SOCS2, SOCS3, SOCS4 and SOCS5 expression in the indicated cells. alpha-Tubulin served as the loading control. ( C and D ) Luciferase assay of cells transfected with pmirGLO-3'UTR reporter of SOCS1 and SOCS3 in miR-196b-5p overexpressing and silencing HCT116 and SW480 cells, respectively. Error bars represent the mean +- SD of three independent experiments. * P < 0.05. ( E and F ) MiRNP IP assay showing the association between miR-196b-5p and SOCS1,SOCS3 transcripts in HCT116 and SW480 cells. Pulldown of IgGantibody served as the negative control. Error bars represent the mean +- SD of three independent experiments. * P < 0.05. ( G ) STAT3 transcriptional activity was assessed by luciferase reporter constructs in the indicated cells. Error bars represent the mean +- SD of three independent experiments. * P < 0.05. ( H ) Western blotting of nuclear STAT3 expression. The nuclear protein p84 was used as the nuclear protein marker.
