Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003248-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003248-M01, RRID:AB_1204498
- Product name
- HPGD monoclonal antibody (M01), clone 1D8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant HPGD.
- Antigen sequence
MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALV
DWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQ
LRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQ
INLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLA
GLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSG
VRLNAICPGFVNTAILESIEKEENMGQYIEYKDHI
KDMIKYYGILDPPLIANGLITLIEDDALNGAIMKI
TTSKGIHFQDYDTTPFQAKTQ- Isotype
- IgG
- Antibody clone number
- 1D8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HPGD expression in transfected 293T cell line by HPGD monoclonal antibody (M01), clone 1D8.Lane 1: HPGD transfected lysate(29 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HPGD is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HPGD transfected lysate using anti-HPGD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HPGD MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HPGD on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
