NB200-179

antibody from Novus Biologicals

Targeting: HPGD SDR36C1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-179

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-179, RRID:AB_10002546

Product name

Rabbit Polyclonal 15-PGDH/HPGD Antibody

Antibody type

Polyclonal

Description

Protein A purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

2 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets.

Corwin C, Nikolopoulou A, Pan AL, Nunez-Santos M, Vallabhajosula S, Serrano P, Babich J, Figueiredo-Pereira ME
Journal of neuroinflammation 2018 Sep 20;15(1):272

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15-hydroxyprostaglandin dehydrogenase (15-PGDH) prevents lipopolysaccharide (LPS)-induced acute liver injury.

Yao L, Chen W, Song K, Han C, Gandhi CR, Lim K, Wu T
PloS one 2017;12(4):e0176106

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A potential anti-tumor effect of leukotriene C4 through the induction of 15-hydroxyprostaglandin dehydrogenase expression in colon cancer cells.

Mehdawi LM, Satapathy SR, Gustafsson A, Lundholm K, Alvarado-Kristensson M, Sjölander A
Oncotarget 2017 May 23;8(21):35033-35047

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COX2/mPGES1/PGE2 pathway regulates PD-L1 expression in tumor-associated macrophages and myeloid-derived suppressor cells.

Prima V, Kaliberova LN, Kaliberov S, Curiel DT, Kusmartsev S
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 31;114(5):1117-1122

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Increased Expression of 15-Hydroxyprostaglandin Dehydrogenase in Spinal Astrocytes During Disease Progression in a Model of Amyotrophic Lateral Sclerosis.

Miyagishi H, Kosuge Y, Takano A, Endo M, Nango H, Yamagata-Murayama S, Hirose D, Kano R, Tanaka Y, Ishige K, Ito Y
Cellular and molecular neurobiology 2017 Apr;37(3):445-452

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Non-canonical WNT5A signaling up-regulates the expression of the tumor suppressor 15-PGDH and induces differentiation of colon cancer cells.

Mehdawi LM, Prasad CP, Ehrnström R, Andersson T, Sjölander A
Molecular oncology 2016 Nov;10(9):1415-1429

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Expression of Prostaglandin E2 Enzymes in the Synovium of Arthralgia Patients at Risk of Developing Rheumatoid Arthritis and in Early Arthritis Patients.

de Hair MJ, Leclerc P, Newsum EC, Maijer KI, van de Sande MG, Ramwadhdoebe TH, van Schaardenburg D, van Baarsen LG, Korotkova M, Gerlag DM, Tak PP, Jakobsson PJ
PloS one 2015;10(7):e0133669

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Resolvin D1 Reduces Emphysema and Chronic Inflammation.

Hsiao HM, Thatcher TH, Colas RA, Serhan CN, Phipps RP, Sime PJ
The American journal of pathology 2015 Dec;185(12):3189-201

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Inhibition of 15-hydroxyprostaglandin dehydrogenase by Helicobacter pylori in human gastric carcinogenesis.

Ryu YM, Myung SJ, Park YS, Yang DH, Song HJ, Jeong JY, Lee SM, Song M, Kim DH, Lee HJ, Park SK, Fink SP, Markowitz SD, Jung KW, Kim KJ, Ye BD, Byeon JS, Jung HY, Yang SK, Kim JH
Cancer prevention research (Philadelphia, Pa.) 2013 Apr;6(4):349-59

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Pioglitazone, a PPARγ agonist, suppresses CYP19 transcription: evidence for involvement of 15-hydroxyprostaglandin dehydrogenase and BRCA1.

Subbaramaiah K, Howe LR, Zhou XK, Yang P, Hudis CA, Kopelovich L, Dannenberg AJ
Cancer prevention research (Philadelphia, Pa.) 2012 Oct;5(10):1183-94

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Prognostic implication of 15-hydroxyprostaglandin dehydrogenase down-regulation in patients with colorectal cancer.

Kang PS, Kim JH, Moon OI, Lim SC, Kim KJ
Journal of the Korean Society of Coloproctology 2012 Oct;28(5):253-8

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Limited effect of anti-rheumatic treatment on 15-prostaglandin dehydrogenase in rheumatoid arthritis synovial tissue.

Gheorghe KR, Sadique S, Leclerc P, Idborg H, Wobst I, Catrina AI, Jakobsson PJ, Korotkova M
Arthritis research & therapy 2012 May 22;14(3):R121

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High-fat diet-induced renal cell apoptosis and oxidative stress in spontaneously hypertensive rat are ameliorated by fenofibrate through the PPARα-FoxO3a-PGC-1α pathway.

Chung HW, Lim JH, Kim MY, Shin SJ, Chung S, Choi BS, Kim HW, Kim YS, Park CW, Chang YS
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2012 Jun;27(6):2213-25

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UV radiation inhibits 15-hydroxyprostaglandin dehydrogenase levels in human skin: evidence of transcriptional suppression.

Judson BL, Miyaki A, Kekatpure VD, Du B, Gilleaudeau P, Sullivan-Whalen M, Mohebati A, Nair S, Boyle JO, Granstein RD, Subbaramaiah K, Krueger JG, Dannenberg AJ
Cancer prevention research (Philadelphia, Pa.) 2010 Sep;3(9):1104-11

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Bile acids inhibit NAD+-dependent 15-hydroxyprostaglandin dehydrogenase transcription in colonocytes.

Miyaki A, Yang P, Tai HH, Subbaramaiah K, Dannenberg AJ
American journal of physiology. Gastrointestinal and liver physiology 2009 Sep;297(3):G559-66

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Involvement of PGE2 and PGDH but not COX-2 in thrombin-induced cortical neuron apoptosis.

Thirumangalakudi L, Rao HV, Grammas P
Neuroscience letters 2009 Mar 13;452(2):172-5

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NAD+-dependent 15-hydroxyprostaglandin dehydrogenase regulates levels of bioactive lipids in non-small cell lung cancer.

Hughes D, Otani T, Yang P, Newman RA, Yantiss RK, Altorki NK, Port JL, Yan M, Markowitz SD, Mazumdar M, Tai HH, Subbaramaiah K, Dannenberg AJ
Cancer prevention research (Philadelphia, Pa.) 2008 Sep;1(4):241-9

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Expression of NAD+ dependent 15-hydroxyprostaglandin dehydrogenase and protection of prostaglandins in human hair follicle.

Michelet JF, Colombe L, Gautier B, Gaillard O, Benech F, Pereira R, Boulle C, Dalko-Csiba M, Rozot R, Neuwels M, Bernard BA
Experimental dermatology 2008 Oct;17(10):821-8

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Repression of 15-hydroxyprostaglandin dehydrogenase involves histone deacetylase 2 and snail in colorectal cancer.

Backlund MG, Mann JR, Holla VR, Shi Q, Daikoku T, Dey SK, DuBois RN
Cancer research 2008 Nov 15;68(22):9331-7

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Repression of prostaglandin dehydrogenase by epidermal growth factor and snail increases prostaglandin E2 and promotes cancer progression.

Mann JR, Backlund MG, Buchanan FG, Daikoku T, Holla VR, Rosenberg DW, Dey SK, DuBois RN
Cancer research 2006 Jul 1;66(13):6649-56

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NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH) behaves as a tumor suppressor in lung cancer.

Ding Y, Tong M, Liu S, Moscow JA, Tai HH
Carcinogenesis 2005 Jan;26(1):65-72

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15-Hydroxyprostaglandin dehydrogenase is down-regulated in colorectal cancer.

Backlund MG, Mann JR, Holla VR, Buchanan FG, Tai HH, Musiek ES, Milne GL, Katkuri S, DuBois RN
The Journal of biological chemistry 2005 Feb 4;280(5):3217-23

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Synergistic induction of the nicotinamide adenine dinucleotide-linked 15-hydroxyprostaglandin dehydrogenase by an androgen and interleukin-6 or forskolin in human prostate cancer cells.

Tong M, Tai HH
Endocrinology 2004 May;145(5):2141-7

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15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-beta-induced suppressor of human gastrointestinal cancers.

Yan M, Rerko RM, Platzer P, Dawson D, Willis J, Tong M, Lawrence E, Lutterbaugh J, Lu S, Willson JK, Luo G, Hensold J, Tai HH, Wilson K, Markowitz SD
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 14;101(50):17468-73

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Monoclonal antibodies that inhibit the enzyme activity of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase.

Tai CL, Mak OT, Arai T, Tai HH
The Biochemical journal 1990 Apr 1;267(1):75-8

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Monoclonal antibodies that inhibit the enzyme activity of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase.

Tai CL, Mak OT, Arai T, Tai HH
The Biochemical journal 1990 Apr 1;267(1):75-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Analysis using the HRP conjugate of NB200-179. Detection of 15-PGDH in LoVo whole cell lysates using NB 200-179. Lane 1 uses a 1:6,000 dilution. Lane 2 uses a 1:1,500 dilution. ECL: 10 second exposure.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Western blot analysis of 15-PGDH in Lovo cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB200-179] - IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis . Image courtesy of an anonymous customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Staining in human fetal membrane tissue. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy and inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) and patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: x250 (a, b), x100 (c, d). Image collected and cropped by CiteAb from the following publication (http://arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH/HPGD) in rheumatoid arthritis synovial tissue and the effects of anti-rheumatic treatment on its expression. Double-immunofluorescence images show staining for 15-PGDH/HPGD-positive (red) cells and cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, and endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: x400. Image collected and cropped by CiteAb from the following publication (http://arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY licence