14941-1-AP

antibody from Invitrogen Antibodies

Targeting: BLMH BH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14941-1-AP

Provider

Invitrogen Antibodies

Product name

BLMH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EDGRLVQFL LMNPANDGGQ WDMLVNIVEK YGVIPKKCFP ESYTTEATRR MNDILNHKMR EFCIRLRNLV HSGATKGEIS ATQDVMMEEI FRVVCICLGN PPETFTWEYR DKDKNYQKIG PITPLEFYRE HVKPLFNMED KICLVNDPRP QHKYNKLYTV EYLSNMVGGR KTLYNNQPID FLKKMVAASI KDGEAVWFGC DVGKHFNSKL GLSDMNLYDH ELVFGVSLKN MNKAERLTFG ESLMTHAMTF TAVSEKDDQD GAFTKWRVEN SWGEDHGHKG YLCMTDEWFS EYVYEVVVDR KHVPEEVLAV LEQEPIILPA WDPMGALAE (128-455 aa encoded by BC003616)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human colon tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14941-1-AP (BLMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using BLMH antibody 14941-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BLMH (IP:14941-1-AP, 4ug; Detection:14941-1-AP 1:1000) with mouse skin tissue lysate 3200ug.