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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- AF4634 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GPR56 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human GPR56 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GPR30, rhGPR114, rhGPR115, rhGPR124, and rhGPR125 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
AAP35975- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Adhesion G protein-coupled receptors are activated by exposure of a cryptic tethered agonist.
GPR56 inhibits melanoma growth by internalizing and degrading its ligand TG2.
Stoveken HM, Hajduczok AG, Xu L, Tall GG
Proceedings of the National Academy of Sciences of the United States of America 2015 May 12;112(19):6194-9
Proceedings of the National Academy of Sciences of the United States of America 2015 May 12;112(19):6194-9
GPR56 inhibits melanoma growth by internalizing and degrading its ligand TG2.
Yang L, Friedland S, Corson N, Xu L
Cancer research 2014 Feb 15;74(4):1022-31
Cancer research 2014 Feb 15;74(4):1022-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GPR56 by Western Blot. Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GPR56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4634) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GPR56 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GPR56 by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and SK-BR-3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for GPR56 at approximately 116 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human GPR56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4634) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GPR56 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Sheep Anti-Human GPR56 Affinity-purified Polyclonal Antibody (Catalog # AF4634) or (B) control antibody (Catalog # 5-001-A), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Mouse anti-Human CD56 APC-conjugated monoclonal antibody (Catalog # FAB2408A). View our protocol for Staining Membrane-associated Proteins.
