Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-24868 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Torc1 Monoclonal Antibody (4G9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4G9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Torc1 in HeLa and Jurkat lysates. Samples were incubated with Torc1 monoclonal antibody (Product # MA5-24868) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Torc1 in HeLa cells. Samples were incubated with Torc1 monoclonal antibody (Product # MA5-24868) at a dilution of 1:300.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DBC1 in HeLa cells. Samples were incubated with DBC1 monoclonal antibody (Product # MA5-24866) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Torc1 in HeLa cells. Samples were incubated with Torc1 monoclonal antibody (Product # MA5-24868) at a dilution of 1:300.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Torc1 in K562 cells, normal mouse IgG (blue line, control). Samples were incubated with Torc1 monoclonal antibody (Product # MA5-24868) at a dilution of 1:50, followed by FITC-conjugated goat anti-mouse IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Torc1 in HeLa cells. Samples were incubated with Torc1 monoclonal antibody (Product # MA5-24868).