11281-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF4G3 eIF4GII

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11281-1-AP

Provider

Invitrogen Antibodies

Product name

EIF4G3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SQVPEHSPV VYGTVESAHL AASTPVTAAS DQKQEEKPKP DPVLKSPSPV LRLVLSGEKK EQEGQTSETT AIVSIAELPL PPSPTTVSSV ARSTIAAPTS SALSSQPIFT TAIDDRCELS SPREDTIPIP SLTSCTETSD PLPTNENDGD ICKKPCSVAP NDIPLVSSTN LINEINGVSE KLSATESIVE IVKQEVLPLT LELEILENPP EEMKLECIPA PITPSTVPSF PPTPPTPPAS PPHTPVIVPA AATTVSSPSA AITVQRVLEE DESIRTCLSE DAKEIQNKIE VEADGQTEEI LDSQNLNSRR SPVPETSNEC (197-515 aa encoded by BC030578)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.47 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11281-1-AP ( EIF4G3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 11281-1-AP (EIF4G3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug.