Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AGR-057-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR97 (ADGRG3) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-GPR97 (ADGRG3) (extracellular) Antibody (#AGR-057), (1:200). 3-4. Anti-GPR97 (ADGRG3) (extracellular) Antibody , preincubated with GPR97 (ADGRG3) (extracellular) Blocking Peptide (BLP-GR057).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate: - 1. Anti-GPR97 (ADGRG3) (extracellular) Antibody (#AGR-057), (1:200). 2. Anti-GPR97 (ADGRG3) (extracellular) Antibody , preincubated with GPR97 (ADGRG3) (extracellular) Blocking Peptide (BLP-GR057).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR97 in rat spinal cord. Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-GPR97 (ADGRG3) (extracellular) Antibody (#AGR-057), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn of the spinal cord region, showed immunoreactivity of GPR97 (green) in neuronal outlines (arrows). B. Pre-incubation of the Antibody with GPR97 (ADGRG3) (extracellular) Blocking Peptide (BLP-GR057), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR97 in rat spinal cord. Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-GPR97 (ADGRG3) (extracellular) Antibody (#AGR-057), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn of the spinal cord region, showed immunoreactivity of GPR97 (green) in neuronal outlines (arrows). B. Pre-incubation of the Antibody with GPR97 (ADGRG3) (extracellular) Blocking Peptide (BLP-GR057), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of GPR97 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-GPR97 (ADGRG3) (extracellular) Antibody (#AGR-057) (2.5μg) + goat- Anti-rabbit-FITC.