Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX85822 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85822, RRID:AB_10725351
- Product name
- SLIT1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Down-regulation of Slit-Robo pathway mediating neuronal cytoskeletal remodeling processes facilitates the antidepressive-like activity of Gastrodia elata Blume.
Lin SH, Chen WC, Lu KH, Chen PJ, Hsieh SC, Pan TM, Chen ST, Sheen LY
Journal of agricultural and food chemistry 2014 Oct 29;62(43):10493-503
Journal of agricultural and food chemistry 2014 Oct 29;62(43):10493-503
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Affinity Purified anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates. The expected molecular weight for SLIT-1 is 168 kD. Approximately 20 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kD -160kD?
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-SLIT1 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody GTX85822 concentration 5 ug/ml.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-SLIT1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody GTX85822 concentration 5 ug/ml.
