Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10222 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10222, RRID:AB_1671686
- Product name
- ATAD5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATAD5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability.
A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions.
Kanellis P, Agyei R, Durocher D
Current biology : CB 2003 Sep 16;13(18):1583-95
Current biology : CB 2003 Sep 16;13(18):1583-95
A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions.
Jenne DE, Tinschert S, Stegmann E, Reimann H, Nürnberg P, Horn D, Naumann I, Buske A, Thiel G
Genomics 2000 May 15;66(1):93-7
Genomics 2000 May 15;66(1):93-7
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates.Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates.After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa.The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown).
