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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX25429 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25429, RRID:AB_423860
- Product name
- ATAD5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot using GeneTex Affinity Purified anti-Elg1 antibody (GTX25429) shows detection of a band ~120 kDa corresponding to human Elg1 in various cell lysates. Lanes contain ~ 5ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown
