MA5-34781

antibody from Invitrogen Antibodies

Targeting: UPF1 HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34781

Provider

Invitrogen Antibodies

Product name

UPF1 Recombinant Rabbit Monoclonal Antibody (JG38-44)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: PC-3M, A431, LOVO, human colon tissue, human prostate cancer tissue, human kidney tissue, mouse brain tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG38-44

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hUPF1 in PC-3M cell lysate. Samples were incubated with hUPF1 monoclonal antibody (Product # MA5-34781), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of hUPF1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with hUPF1 monoclonal antibody (Product # MA5-34781), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of hUPF1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with hUPF1 monoclonal antibody (Product # MA5-34781), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of hUPF1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with hUPF1 monoclonal antibody (Product # MA5-34781), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hUPF1 in paraffin-embedded mouse brain tissue. Samples were incubated with hUPF1 monoclonal antibody (Product # MA5-34781), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hUPF1 in paraffin-embedded human prostate cancer tissue. Samples were incubated with hUPF1 monoclonal antibody (Product # MA5-34781), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hUPF1 in paraffin-embedded human kidney tissue. Samples were incubated with hUPF1 monoclonal antibody (Product # MA5-34781), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hUPF1 in paraffin-embedded human colon tissue. Samples were incubated with hUPF1 monoclonal antibody (Product # MA5-34781), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of hUPF1 in PC-3M cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with hUPF1 monoclonal antibody (Product # MA5-34781) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.