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Learn23379-1-AP
antibody from Invitrogen Antibodies
Targeting: UPF1
HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 23379-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UPF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EAANVEKIT TKLLKAGAKP DQIGIITPYE GQRSYLVQYM QFSGSLHTKL YQEVEIASVD AFQGREKDFI ILSCVRANEH QGIGFLNDPR RLNVALTRAR YGVIIVGNPK ALSKQPLWNH LLNYYKEQKV LVEGPLNNLR ESLMQFSKPR KLVNTINPGA RFMTTAMYDA REAIIPGSVY DRSSQGRPSS MYFQTHDQIG MISAGPSHVA AMNIPIPFNL VMPPMPPPGY FGQANGPAAG RGTPKGKTGR GGRQKNRFGL PGPSQTNLPN SQASQDVASQ PFSQGALTQG YISMSQPSQM SQPGLSQPEL SQDSYLGDEF KSQIDVALSQ DSTYQGERAY QHGGVTGLSQ Y (780-1129 aa encoded by BC039817)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 23379-1-AP (UPF1 antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using UPF1 antibody 23379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 23379-1-AP ( UPF1 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 23379-1-AP ( UPF1 Antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-UPF1 (IP:23379-1-AP, 4ug; Detection:23379-1-AP 1:500) with Raji cells lysate 2000ug.
