H00007357-M03

antibody from Abnova Corporation

Targeting: UGCG GCS

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007357-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007357-M03, RRID:AB_1716783

Product name

UGCG monoclonal antibody (M03), clone 1E5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant UGCG.

Antigen sequence

TRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLI
NNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLL
GKYPNVDARLFIGGKKVGINPKINNLMPG


Isotype

IgG

Antibody clone number

1E5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Fenretinide sensitizes multidrug-resistant human neuroblastoma cells to antibody-independent and ch14.18-mediated NK cell cytotoxicity.

Shibina A, Seidel D, Somanchi SS, Lee DA, Stermann A, Maurer BJ, Lode HN, Reynolds CP, Huebener N
Journal of molecular medicine (Berlin, Germany) 2013 Apr;91(4):459-72

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DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells.

Haynes TA, Filippov V, Filippova M, Yang J, Zhang K, Duerksen-Hughes PJ
Biochimica et biophysica acta 2012 Jul;1821(7):943-53

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

UGCG monoclonal antibody (M03), clone 1E5. Western Blot analysis of UGCG expression in HL-60.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UGCG is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol