AP09297PU-N

antibody from Acris Antibodies GmbH

Targeting: MARK2 EMK1, PAR-1, PAR-1B, Par1b

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09297PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09297PU-N, RRID:AB_2035736

Product name

anti MARK2 pThr595

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.6 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-MARK2 pT595 antibody shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead). HEK293 cells were transfected with pCMV-3xFlag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3xFlag-MARK2 only (lane 4). Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with anti-MARK2 pT595. An untransfected HEK293 cell lysate was used as a negative control (lane 2). MW markers are shown for size comparison (lane 1). PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phosphospecific staining are observed in lysates from cells transfected with both MARK2 and PKCzeta.