P02117

antibody from Boster Biological Technology

Targeting: MARK2 EMK1, PAR-1, PAR-1B, Par1b

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

P02117

Provider

Boster Biological Technology

Product name

Anti-Phospho-MARK2 T595 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for MARK2 detection. Host: Rabbit.Size: 100μg. Tested applications: IF. Reactive species: Human. MARK2 information: Molecular Weight: 87911 MW; Subcellular Localization: Cell membrane; Peripheral membrane protein. Cytoplasm. Lateral cell membrane. Cytoplasm, cytoskeleton. Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm; Tissue Specificity: High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of MARK2 expression in wild type Jurkat cells (lane 1) and wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation) (lane 2). MARK2 at 83KD was detected using rabbit anti-MARK2 pT595 Antigen Affinity purified polyclonal antibody (Catalog # P02117) at 1:500.

Additional image