PAB28615

antibody from Abnova Corporation

Targeting: CGRRF1 CGR19, RNF197

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28615

Provider

Abnova Corporation

Product name

CGRRF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CGRRF1.

Antigen sequence

IYDIISMVSVIHIPDRTYKLSCRILYQYLLLAQGQ
FHDLKQLFMSANNNFTPSNNSSSEEKNTDRSLLEK
VGLSESEVEPSEENSKDCVVCQNGTVNWVLLPCRH
TCLCDGCVKYFQQCPMCRQFVQESFALCSQKEQDK
DKPK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp, Lane 3: Human plasma (IgG/HSA depleted), Lane 4: Human liver tissue with CGRRF1 polyclonal antibody (Cat # PAB28615) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with CGRRF1 polyclonal antibody (Cat # PAB28615) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with CGRRF1 polyclonal antibody (Cat # PAB28615) shows strong cytoplasmic positivity in hepatocytes at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)