H00005094-M07

antibody from Invitrogen Antibodies

Targeting: PCBP2 hnRNP-E2, HNRNPE2, HNRPE2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005094-M07

Provider

Invitrogen Antibodies

Product name

PCBP2 Monoclonal Antibody (5F12)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: MDTGVIEGGL NVTLTIRLLM HGKEVGSIIG KKGESVKKMR EESGARINIS EGNCPERIIT LAGPTNAIFK AFAMIIDKLE EDISSSMTNS TAASRPPVTL RLVVPASQCG SLIGKGGCKI KEIRESTGAQ VQVAGDMLPN STERAITIAG IPQSIIECVK QICVVMLESP PKGVTIPYRP KPSSSPVIFA GGQDRYSTGS DSASFPHTTP SMCLNPDLEG PPLEAYTIQG QYAIPQPDLT KLHQLAMQQS HFPMTHGNTG FSGIESSSPE VKGYWAGLDA SAQTTSHELT IPNDLIGCII GRQGAKINEI RQMSGAQIKI ANPVEGSTDR QVTITGSAAS ISLAQYLINV RLSSETGGMG SS

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5F12

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi (Cat # H00005094-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody (M07), clone 5F12 (Cat # H00005094-M07). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PCBP2 monoclonal antibody (M07), clone 5F12 Western Blot analysis of PCBP2 expression in K-562 (Cat # L009V1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12.Lane 1: PCBP2 transfected lysate(38.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PCBP2 is approximately 0.1 ng/mL as a capture antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PCBP2 is approximately 0.1 ng/mL as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell. Antibody concentration is 10 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. Antibody concentration is 3 µg/mL.