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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-30116 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, K562, NCI-H929. Predicted reactivity: Mouse (100%), Rat (97%), Pig (91%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.03 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Comprehensive profiling of mRNA splicing indicates that GC content signals altered cassette exon inclusion in Ewing sarcoma.
Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent Manner.
Graham GT, Selvanathan SP, Zöllner SK, Stahl E, Shlien A, Caplen NJ, Üren A, Toretsky JA
NAR cancer 2022 Mar;4(1):zcab052
NAR cancer 2022 Mar;4(1):zcab052
Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent Manner.
Zheng Q, Zhang X, Yang H, Xie J, Xie Y, Chen J, Yu C, Zhong C
Viruses 2019 Oct 8;11(10)
Viruses 2019 Oct 8;11(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCBP2 using 30 µg of A) Jurkat (B) Raji (C) K562 and D) NCI-H929 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PCBP2 polyclonal antibody (Product # PA5-30116) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP2 was performed by separating 30 µg of various whole cell extracts by 12 % SDS-PAGE. Proteins were transferred to a membrane and probed with a PCBP2 Polyclonal Antibody (Product # PA5-30116) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PCBP2 was achieved by transfecting HeLa cells with PCBP2 specific siRNAs (Silencer® select Product # s10097, s10099). Western blot analysis (Fig. a) was performed using nuclear extracts from the PCBP2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PCBP2 Polyclonal Antibody (Product # PA5-30116, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PCBP2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), Hep G2 (Lane 3), RAW 264.7 (Lane 4), Raji (Lane 5) and Jurkat (Lane 6). The blot was probed with Anti- PCBP2 Polyclonal Antibody (Product # PA5-30116, 1µg/ml) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to NDUFS1 was detected across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PCBP2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PCBP2 Polyclonal Antibody (Product # PA5-30116) diluted at 1:750. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCBP2 was performed in paraffin-embedded mouse brain tissue using PCBP2 Polyclonal Antibody (Product # PA5-30116) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human muscle, using PCBP2 (Product # PA5-30116) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 EMCV IRES inhibited the expression of transgene on the translational level. ( A ) HEK293 cells were transduced with rAAV6-CMVp- gfp at 10,000 vgs/cell. The relative rAAV6 genome content was detected by qPCR using GFP primers and ITR primers. ( B ) K562 and ( C ) HEK293 cells were transduced with rAAV6-CMVp- gfp and rAAV6-CMVp-EMCV IRES- gfp at 10,000 vgs/cell. Total DNA and RNA were isolated at 4 days post-transduction for qPCR. Transgene expression was detected by fluorescence microscopy at 72 hours post-transduction. (D) Western blot of total cell extracts (lysate) from HEK293 cells and K562 cells after rAAV6-CMVp- gfp or rAAV6-CMVp-EMCV IRES- gfp infection for Gemin5, PTBP1, and PCBP2 expression.
