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- References [9]
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- Western blot [4]
- Immunohistochemistry [7]
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- Product number
- NBP1-89631 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89631, RRID:AB_11035753
- Product name
- Rabbit Polyclonal Lipolysis Stimulated Lipoprotein Receptor Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Lipolysis Stimulated Lipoprotein Receptor antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Epithelial barrier dysfunction and cell migration induction via JNK/cofilin/actin by angubindin-1.
HMGB1-downregulated angulin-1/LSR induces epithelial barrier disruption via claudin-2 and cellular metabolism via AMPK in airway epithelial Calu-3 cells.
Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells.
Guanylate binding protein-1-mediated epithelial barrier in human salivary gland duct epithelium.
Histone deacetylase inhibition prevents cell death induced by loss of tricellular tight junction proteins in temperature-sensitive mouse cochlear cells.
Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.
LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation.
Poly(I:C) induced microRNA-146a regulates epithelial barrier and secretion of proinflammatory cytokines in human nasal epithelial cells.
The role of lipolysis stimulated lipoprotein receptor in breast cancer and directing breast cancer cell behavior.
Konno T, Kohno T, Kikuchi S, Shimada H, Satohisa S, Saito T, Kondoh M, Kojima T
Tissue barriers 2020;8(1):1695475
Tissue barriers 2020;8(1):1695475
HMGB1-downregulated angulin-1/LSR induces epithelial barrier disruption via claudin-2 and cellular metabolism via AMPK in airway epithelial Calu-3 cells.
Kodera Y, Chiba H, Konno T, Kohno T, Takahashi H, Kojima T
Biochemical and biophysical research communications 2020 Jun 25;527(2):553-560
Biochemical and biophysical research communications 2020 Jun 25;527(2):553-560
Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells.
Konno T, Kohno T, Kikuchi S, Shimada H, Satohisa S, Takano K, Saito T, Kojima T
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2020 Jan;68(1):59-72
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2020 Jan;68(1):59-72
Guanylate binding protein-1-mediated epithelial barrier in human salivary gland duct epithelium.
Konno T, Takano K, Kaneko Y, Kakuki T, Nomura K, Yajima R, Kakiuchi A, Kohno T, Himi T, Kojima T
Experimental cell research 2018 Oct 1;371(1):31-41
Experimental cell research 2018 Oct 1;371(1):31-41
Histone deacetylase inhibition prevents cell death induced by loss of tricellular tight junction proteins in temperature-sensitive mouse cochlear cells.
Takano K, Kakuki T, Kaneko Y, Kohno T, Kikuchi S, Himi T, Kojima T
PloS one 2017;12(8):e0182291
PloS one 2017;12(8):e0182291
Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.
Shimada H, Satohisa S, Kohno T, Konno T, Takano KI, Takahashi S, Hatakeyama T, Arimoto C, Saito T, Kojima T
Oncology letters 2017 Dec;14(6):6776-6782
Oncology letters 2017 Dec;14(6):6776-6782
LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation.
Sohet F, Lin C, Munji RN, Lee SY, Ruderisch N, Soung A, Arnold TD, Derugin N, Vexler ZS, Yen FT, Daneman R
The Journal of cell biology 2015 Mar 16;208(6):703-11
The Journal of cell biology 2015 Mar 16;208(6):703-11
Poly(I:C) induced microRNA-146a regulates epithelial barrier and secretion of proinflammatory cytokines in human nasal epithelial cells.
Miyata R, Kakuki T, Nomura K, Ohkuni T, Ogasawara N, Takano K, Konno T, Kohno T, Sawada N, Himi T, Kojima T
European journal of pharmacology 2015 Aug 15;761:375-82
European journal of pharmacology 2015 Aug 15;761:375-82
The role of lipolysis stimulated lipoprotein receptor in breast cancer and directing breast cancer cell behavior.
Reaves DK, Fagan-Solis KD, Dunphy K, Oliver SD, Scott DW, Fleming JM
PloS one 2014;9(3):e91747
PloS one 2014;9(3):e91747
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Simple Western lane view shows a specific band for LSR in 0.2 mg/ml of RT-4 (left), A431 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Electropherogram image(s) of corresponding Simple Western lane view. Lipolysis Stimulated Lipoprotein Receptor antibody was used at 1:20 dilution on RT-4 and A431 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell line CACO-2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human endometrium shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human stomach shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human placenta shows strong membranous positivity in trophoblast.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human liver shows moderate membranous positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human kidney shows weak positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining in human stomach and kidney tissues . Corresponding LSR RNA-seq data are presented for the same tissues.
