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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- OAAB03420 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- AGR2 antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected with the AGR2 gene (Lane 2) (Origene Technologies).
- Sample type
- lysatestransiently transfected with the AGR2 gene
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-AGR2 Antibody (Center) (RB13857) in NCI-H460 cell lysates (35ug/lane). AGR2(arrow) was detected using the purified Pab (1:60 dilution).
- Sample type
- NCI-H460 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human lung carcinoma tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
