SM7216A

antibody from Acris Antibodies GmbH

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

SM7216A

Provider

Acris Antibodies GmbH

Product name

anti AGR2 (55-72)

Antibody type

Polyclonal

Antigen

Synthetic peptides corresponding to amino acids 55-72 of human AGR2 (Genbank accession no. NP_006399.1)

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.1 mg

Concentration

0.5 mg/ml

References

Submitted references

Loss of anterior gradient 2 (Agr2) expression results in hyperplasia and defective lineage maturation in the murine stomach.

Gupta A, Wodziak D, Tun M, Bouley DM, Lowe AW
The Journal of biological chemistry 2013 Feb 8;288(6):4321-33

---

AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia.

Norris AM, Gore A, Balboni A, Young A, Longnecker DS, Korc M
Oncogene 2013 Aug 15;32(33):3867-76

---

The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development.

Verma S, Salmans ML, Geyfman M, Wang H, Yu Z, Lu Z, Zhao F, Lipkin SM, Andersen B
Developmental biology 2012 Sep 15;369(2):249-60

---

SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production.

Chen G, Korfhagen TR, Xu Y, Kitzmiller J, Wert SE, Maeda Y, Gregorieff A, Clevers H, Whitsett JA
The Journal of clinical investigation 2009 Oct;119(10):2914-24

---

New asthma biomarkers: lessons from murine models of acute and chronic asthma.

Di Valentin E, Crahay C, Garbacki N, Hennuy B, Guéders M, Noël A, Foidart JM, Grooten J, Colige A, Piette J, Cataldo D
American journal of physiology. Lung cellular and molecular physiology 2009 Feb;296(2):L185-97

---

Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation.

Sitek B, Sipos B, Alkatout I, Poschmann G, Stephan C, Schulenborg T, Marcus K, Lüttges J, Dittert DD, Baretton G, Schmiegel W, Hahn SA, Klöppel G, Meyer HE, Stühler K
Journal of proteome research 2009 Apr;8(4):1647-56

---

The protein disulfide isomerase AGR2 is essential for production of intestinal mucus.

Park SW, Zhen G, Verhaeghe C, Nakagami Y, Nguyenvu LT, Barczak AJ, Killeen N, Erle DJ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):6950-5

---

The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation.

Wang Z, Hao Y, Lowe AW
Cancer research 2008 Jan 15;68(2):492-7

---

Proteomic changes during intestinal cell maturation in vivo.

Chang J, Chance MR, Nicholas C, Ahmed N, Guilmeau S, Flandez M, Wang D, Byun DS, Nasser S, Albanese JM, Corner GA, Heerdt BG, Wilson AJ, Augenlicht LH, Mariadason JM
Journal of proteomics 2008 Dec 2;71(5):530-46

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of AGR2 using AGR2 antibody: MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1/100. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of AGR2 using AGR2 antibody: Formalin-fixed, paraffin-embedded normal colon tissue probed with AGR2 antibody at 1/100 dilution.