Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX130229 - Provider product page
- Provider
- GeneTex
- Product name
- Anterior Gradient 2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AGR2 antibody detects AGR2 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 15% SDS-PAGE, and the membrane was blotted with AGR2 antibody (GTX130229) at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AGR2 protein stained by AGR2 antibody (GTX130229) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of AGR2 protein from MCF-7 whole cell extracts using 5 £gg of AGR2 antibody (GTX130229).Western blot analysis was performed using AGR2 antibody (GTX130229).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AGR2 antibody detects AGR2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. AGR2 antibody (GTX130229) diluted at 1:500.
