12275-1-AP

antibody from Invitrogen Antibodies

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12275-1-AP

Provider

Invitrogen Antibodies

Product name

AGR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SW 1990 cells were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human stomach tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat stomach tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12275-1-AP ( AGR2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12275-1-AP ( AGR2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 A549 cells were stained with 0.20 ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 A549 cells were stained with 0.20 ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AGR2 (IP: 12275-1-AP, 4 µg; Detection: 12275-1-AP 1:1000) with SW 1990 cells lysate 2400 µg.