Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [4]
- Flow cytometry [3]
- Other assay [1]
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Validation data
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- Product number
- 12275-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AGR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SW 1990 cells were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human stomach tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat stomach tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12275-1-AP ( AGR2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12275-1-AP ( AGR2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A549 cells were stained with 0.20 ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A549 cells were stained with 0.20 ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-AGR2 (IP: 12275-1-AP, 4 µg; Detection: 12275-1-AP 1:1000) with SW 1990 cells lysate 2400 µg.