HPA020382

antibody from Atlas Antibodies

Targeting: INIP C9orf80, HSPC043, hSSBIP1, MISE, SOSS-C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA020382

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA020382, RRID:AB_1845840

Product name

Anti-INIP

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KEKRKLLMQNQSSTNHPGASIALSRPSLNKDFRDH
AEQQHIAAQQKAALQHAHAHSSGYFITQDSAFGNL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and INIP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412020).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using HPA020382 antibody. Corresponding INIP RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human thyroid gland shows nuclear positivity in glandular cells.

Sample type

HUMAN