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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-17045 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EBP50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 10 µg/mL
- Storage
- -20°C
Submitted references Clinical significance of EBP50 overexpression assessed by quantum dot analysis in gastric cancer.
Lv XG, Lei XF, Ji MY, Guo XF, Wang J, Dong WG
Oncology letters 2013 Jun;5(6):1844-1848
Oncology letters 2013 Jun;5(6):1844-1848
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NHERF1 in extracts from MCF-7 and T-47D cells using NHERF1 polyclonal antibody (Product # PA5-17045).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # PA5-17045) and a 50 kDa band corresponding to EBP50 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of MCF-7(Lane 1), T-47D ( Lane 2), SK-BR-3 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), and PC-12 ( Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NHERF1 in MCF-7 cells using a NHERF1 polyclonal antibody (Product # PA5-17045) (green) showing staining at the apical membrane. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Immunohistochemistry showing EBP50 staining in GC. Sample of (A) negative EBP50 protein expression, (B) a positive case scoring 1+; (C) a positive case scoring 2+, and (D) a positive case scoring 3+ [magnification, x400; objective lens; Streptomyces avidin-peroxidase connection (SP) method]. EBP50, ezrin-radixin-moesin-binding phosphoprotein 50; GC, gastric cancer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Analysis of EBP50 in advanced gastric cancer using quantum dots (QDs). (A) Sample negative for EBP50 protein expression; (B) a positive case scoring 1+; (C) a positive case scoring 2+; and (D) a positive case scoring 3+ (magnification, x400; objective lens). EBP50, ezrin-radixin-moesin-binding phosphoprotein 50.
