Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LF-MA0209 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TRBP Monoclonal Antibody (46D1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is 293T cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 46D1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references TARBP2-Enhanced Resistance during Tamoxifen Treatment in Breast Cancer.
A Rapid Induction Mechanism for Lin28a in Trophic Responses.
Trbp Is Required for Differentiation of Myoblasts and Normal Regeneration of Skeletal Muscle.
Trbp regulates heart function through microRNA-mediated Sox6 repression.
Wang MY, Huang HY, Kuo YL, Lo C, Sun HY, Lyu YJ, Chen BR, Li JN, Chen PS
Cancers 2019 Feb 12;11(2)
Cancers 2019 Feb 12;11(2)
A Rapid Induction Mechanism for Lin28a in Trophic Responses.
Amen AM, Ruiz-Garzon CR, Shi J, Subramanian M, Pham DL, Meffert MK
Molecular cell 2017 Feb 2;65(3):490-503.e7
Molecular cell 2017 Feb 2;65(3):490-503.e7
Trbp Is Required for Differentiation of Myoblasts and Normal Regeneration of Skeletal Muscle.
Ding J, Nie M, Liu J, Hu X, Ma L, Deng ZL, Wang DZ
PloS one 2016;11(5):e0155349
PloS one 2016;11(5):e0155349
Trbp regulates heart function through microRNA-mediated Sox6 repression.
Ding J, Chen J, Wang Y, Kataoka M, Ma L, Zhou P, Hu X, Lin Z, Nie M, Deng ZL, Pu WT, Wang DZ
Nature genetics 2015 Jul;47(7):776-83
Nature genetics 2015 Jul;47(7):776-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of T-47D (Lane 1), Ramos (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), PC-3 (Lane 6) and DU 145 (Lane 7). The blots were probed with Anti-TRBP Mouse Monoclonal Antibody (Product # LF-MA0209, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 39 kDa band corresponding to TRBP was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with overnight wet transfer system. The membrane was probed with the relevant primary and secondary Antibody. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).