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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF7337 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat STI1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, rat STI1 in Western blots and detects recombinant human STI1 in direct ELISAs.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P31948- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Exploitation of the Leishmania exosomal pathway by Leishmania RNA virus 1.
Atayde VD, da Silva Lira Filho A, Chaparro V, Zimmermann A, Martel C, Jaramillo M, Olivier M
Nature microbiology 2019 Apr;4(4):714-723
Nature microbiology 2019 Apr;4(4):714-723
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat STI1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat STI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7337) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STI1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
