HPA021794

antibody from Atlas Antibodies

Targeting: PRPF4 HPRP4, HPRP4P, PRP4, Prp4p, SNRNP60

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA021794

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA021794, RRID:AB_1855772

Product name

Anti-PRPF4

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

QATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLA
KGESGILGKDGLKAGIEAGNINITSGEVFEIEEHI
SERQAEVLAEFERRKRARQINVSTDDS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-PRPF4 antibody HPA021794 (A) shows similar pattern to independent antibody HPA022248 (B).

Antibody #2 product nr

HPA022248

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, liver, lymph node and testis using Anti-PRPF4 antibody HPA021794 (A) shows similar protein distribution across tissues to independent antibody HPA022248 (B).

Antibody #2 product nr

HPA022248

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-PRPF4 antibody HPA021794.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-PRPF4 antibody HPA021794.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-PRPF4 antibody HPA021794.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-PRPF4 antibody HPA021794.

Sample type

HUMAN