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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-54511 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRPF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QATKTKAPDD LVAPVVKKPH IYYGSLEEKE RERLAKGESG ILGKDGLKAG IEAGNINITS GEVFEIEEHI SERQAEVLAE FERRKRARQI NVSTDDS
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRPF4 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PRPF4 Polyclonal Antibody (Product # PA5-54511).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRPF4 using PRPF4 Polyclonal Antibody (Product # PA5-54511) (A) shows similar pattern to an independent PRPF4 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PRPF4 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PRPF4 Polyclonal Antibody (Product # PA5-54511).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PRPF4 in human cell line U-2 OS using a PRPF4 Polyclonal Antibody (Product # PA5-54511) shows localization to nuclear speckles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRPF4 in human lymph node using PRPF4 Polyclonal Antibody (Product # PA5-54511).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRPF4 in human cerebral cortex using PRPF4 Polyclonal Antibody (Product # PA5-54511).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRPF4 in human liver using PRPF4 Polyclonal Antibody (Product # PA5-54511).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRPF4 in human testis using PRPF4 Polyclonal Antibody (Product # PA5-54511).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRPF4 in human cerebral cortex, liver, lymph node and testis using PRPF4 Polyclonal Antibody (Product # PA5-54511) (A) shows similar protein distribution across tissues to an independent PRPF4 Polyclonal Antibody (B).
